FastQCFastQC Report
Mon 6 Feb 2017
SRR2078265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553400
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34800.034.7823831
GGTATCA17700.033.9006041
TCAACGC42750.027.908014
CAACGCA42850.027.841795
ATCAACG44150.027.0225163
TATCAAC45000.026.510532
AACGCAG45300.026.2531916
ACGCAGA53100.022.1379837
GATCCCG6500.021.76024459
CGCAGAG57600.020.1103948
GTACATG67600.020.0993021
AGAGTAC53900.020.02449211
CGTGATC6850.019.64232456
CGTGCCG4400.019.59265310
CGCCCAA5150.019.43708650
CCGTGAT7100.019.43546555
TACATGG69400.019.2764532
ACGCCCA5050.019.13020149
ATCCCGG7600.019.06503160
CAGAGTA60500.018.29597710
Produced by FastQC (version 0.11.5)