FastQCFastQC Report
Mon 6 Feb 2017
SRR2078265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553400
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.040.7109341
GTATCAA49650.040.1417431
TATCAAC70350.027.9771612
TCAACGC71600.027.387094
ATCAACG72100.027.2452163
CAACGCA72000.027.0905845
AACGCAG77450.025.4079346
GCGATAC3950.022.7087914
ACGCAGA88050.022.0415347
TGCGATA4200.021.35707713
CGCAGAG91350.021.1456328
AGAGTAC88850.021.08434711
GTACATG67600.020.0995791
GAGTACT59950.019.79643612
GTGCGAT4950.019.5150912
CAGAGTA97900.019.38196210
TACATGG69750.019.2258762
GCAGAGT100300.018.846079
CCTATAC5150.018.8361993
AGTACTT63750.018.72464813
Produced by FastQC (version 0.11.5)