FastQCFastQC Report
Mon 6 Feb 2017
SRR2078264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873796
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.035.4585041
GTATCAA40300.033.3757171
TCAACGC49850.025.5257994
ATCAACG50200.025.4151943
CAACGCA50150.025.3691225
TATCAAC51650.025.2335872
AACGCAG53750.023.5963786
ACGCAGA60900.020.6576927
CGCAGAG64750.019.2188158
AGAGTAC62800.018.39514711
GTACATG74350.018.0906721
TTAGTCG1351.5903494E-417.87064639
GTATAGA7150.017.364711
TACGCTC1602.9061164E-517.28207434
TACATGG77450.017.139452
GAGTACT27500.016.92852812
CAGAGTA69900.016.82268510
TATACGA1250.001949624316.5497425
GCAGAGT74100.015.91570959
CGCACGA3058.3491614E-1015.86966835
Produced by FastQC (version 0.11.5)