FastQCFastQC Report
Mon 6 Feb 2017
SRR2078264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873796
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.041.6981851
GTATCAA58000.039.8516541
TATCAAC79800.028.6947732
ATCAACG81350.028.1856963
CAACGCA81450.027.9809935
TCAACGC81750.027.9630494
AACGCAG85600.026.9076776
CGAATCG3450.022.98784837
ACGCAGA101650.022.3978947
CGCAGAG105050.021.540898
GTACATG75400.021.0495411
AGAGTAC103950.020.44467411
TACATGG75700.020.4100112
GAGTACT70650.020.26526512
CGTTATG4250.019.47206167
TATGCGA1258.7472596E-519.3198812
CAGAGTA113150.019.20922910
AGTACTT74950.019.1489813
ACATGGG80650.018.81043
GCAGAGT117550.018.7527249
Produced by FastQC (version 0.11.5)