FastQCFastQC Report
Mon 6 Feb 2017
SRR2078263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471751
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.035.7351881
GTATCAA38600.034.478171
TCAACGC47950.027.1804264
CAACGCA48400.026.8553855
ATCAACG49700.026.3599783
TATCAAC50350.026.2914412
AACGCAG51450.025.5901416
GCGATAC3150.022.99010514
ACGCAGA59050.022.005187
GTACATG69450.021.7443331
CGCAGAG62650.020.6327748
TACATGG71750.020.3716682
TGCGATA3400.020.28538713
CGAATCG2750.020.10360737
AGAGTAC59150.019.29769711
ACATGGG73150.019.1350483
GTATTAG5100.018.9291921
GTGCGAT3850.018.81008512
GAGTACT28350.018.73267712
ATAGGCG3001.8189894E-1218.39879456
Produced by FastQC (version 0.11.5)