FastQCFastQC Report
Mon 6 Feb 2017
SRR2078263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471751
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.040.9733281
GTATCAA51850.039.6969261
TATCAAC70600.029.150042
ATCAACG73000.028.3294583
TCAACGC73250.028.2800624
CAACGCA74100.027.8154165
AACGCAG77400.026.7637526
ACGCAGA89450.022.705567
GAGTACT59250.021.66281312
CGCAGAG93600.021.5606168
GTACATG69350.021.4353221
AGAGTAC90950.021.13064411
TACATGG71500.020.448612
AGTACTT63200.020.25429313
CAGAGTA97750.020.04895810
GCAGAGT100300.019.98289
CCGGTAT4050.019.58176458
GTACTTT65450.019.55800214
ACATGGG74300.019.115333
TACGCTC1451.2713701E-519.02473334
Produced by FastQC (version 0.11.5)