FastQCFastQC Report
Mon 6 Feb 2017
SRR2078262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060042
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.038.491561
GTATCAA37600.033.3778531
TATCAAC47750.025.8446162
TCAACGC47800.025.6062094
ATCAACG48500.025.447433
CAACGCA49150.025.0419675
AACGCAG50300.024.2532026
GTACATG74050.021.1851311
ACTCGAC3300.020.91826246
CGACCCG1004.356352E-420.6859265
GCGCACT3350.020.58351142
TACATGG76400.020.2136672
ACGCAGA60100.019.554447
CGTATAG900.00575688819.1545681
TCGACAG3450.019.00371248
ACATGGG80250.018.988033
CGCAGAG61650.018.897398
CTCGACA3550.018.47243947
CGACAGG3950.018.37754234
AGAGTAC60600.018.26499211
Produced by FastQC (version 0.11.5)