FastQCFastQC Report
Mon 6 Feb 2017
SRR2078262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060042
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.1116482091141831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.044.195961
GTATCAA54400.042.70861
TATCAAC77050.030.0084932
ATCAACG77700.029.7077713
TCAACGC78450.029.3795744
CAACGCA80000.028.853675
AACGCAG83150.027.8022776
ACGCAGA95650.024.0109447
CGCAGAG98200.023.2179788
AGAGTAC97100.022.38249411
GAGTACT66600.022.01361312
AACGTCG953.0733278E-421.78258122
CAGAGTA102700.021.56511110
GTACATG66550.021.3115311
GCAGAGT107000.021.017829
AGTACTT69600.020.91605613
TACATGG69100.020.5180592
GTGGTAT7300.020.4260771
GTACTTT71400.020.24381814
ACATGGG71500.019.5351093
Produced by FastQC (version 0.11.5)