FastQCFastQC Report
Mon 6 Feb 2017
SRR2078261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161772
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.034.9935651
GTATCAA27100.031.6867771
TCAACGC32250.025.8733794
CAACGCA33150.025.2737775
ATCAACG33900.024.8157523
TATCAAC35450.023.825772
AACGCAG35600.023.8172516
GTACATG59600.022.161571
TACATGG60650.021.2019182
ACATGGG61550.019.6054733
ACGCAGA42400.019.2676737
CGCAGAG44450.018.381198
CGTGCCG2651.1823431E-1018.21670310
CATGGGG42550.017.1792344
GCACCGT4900.016.8819166
AGAGTAC42200.016.83225811
GGCGTAC1653.8617123E-516.7183613
CGAATTA1452.723323E-416.6555666
GTGTAGC8600.016.0401921
CAGAGTA45800.015.96092210
Produced by FastQC (version 0.11.5)