FastQCFastQC Report
Mon 6 Feb 2017
SRR2078261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161772
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40200.039.171591
GGTATCA17250.038.8069571
TCAACGC56250.027.9180264
TATCAAC56250.027.8609312
ATCAACG56650.027.6597063
CAACGCA56500.027.6104225
TACCGCG756.190905E-527.5836629
AACGCAG60400.025.7702396
CGTGCCG1801.2732926E-1124.91574710
ACCGTAT2250.024.5284548
GAGTACT43200.022.12071212
ACGCAGA70400.022.0292537
CGCAGAG71250.021.7367298
AGAGTAC69800.020.95624711
GTACATG57250.020.2960131
AGTACTT47700.020.17850113
CAGAGTA74600.019.70034610
TACATGG59400.019.437372
GTACTTT48550.019.25675614
GCAGAGT79150.019.087369
Produced by FastQC (version 0.11.5)