FastQCFastQC Report
Mon 6 Feb 2017
SRR2078260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579196
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.035.2050171
GGTATCA17750.032.046031
ATCAACG41900.026.7392673
TCAACGC42450.026.3116154
CAACGCA43200.025.9336075
TATCAAC44050.025.5877132
AACGCAG46100.024.5189256
ACGCAGA53950.020.6335167
CGCAGAG55450.020.1398498
CTATCGT900.00572742219.1718146
GAGTACT25200.019.02082412
GTACATG64850.018.5525681
AGAGTAC54100.018.35736511
TACATGG64150.018.053952
CAGAGTA59050.017.51928710
ACATGGG65300.017.4741083
CGTGCCG3003.6379788E-1117.241910
GCAGAGT63600.016.7539229
GCGATAC2904.2382453E-1016.6473514
GTACTTT29400.016.42085514
Produced by FastQC (version 0.11.5)