FastQCFastQC Report
Mon 6 Feb 2017
SRR2078260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579196
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43000.038.7860571
GGTATCA19400.036.1034431
ATCAACG58400.028.7217853
TCAACGC58250.028.6767544
CAACGCA58750.028.3147185
TATCAAC59850.028.0297582
AACGCAG62500.026.6158356
TGCGATA2500.023.46004713
GCGATAC2500.023.46004714
ACGCAGA73750.022.3854737
CGCAGAG75750.021.8129238
GAGTACT49800.020.50606512
GTACATG61500.020.0712241
AGAGTAC76250.019.8629911
TACATGG61450.019.2904512
CAGAGTA82150.019.10837610
GCAGAGT86050.018.6808249
AGTACTT56000.018.54378713
TCGATCG1702.446368E-618.25553541
GTACTTT56000.018.2357514
Produced by FastQC (version 0.11.5)