FastQCFastQC Report
Mon 6 Feb 2017
SRR2078259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295976
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43500.037.020661
GGTATCA20800.036.1417921
TCAACGC55850.028.3391694
ATCAACG55950.028.2848113
CAACGCA56450.028.1576675
TATCAAC57600.027.7115822
TATCGCG500.00775412627.587139
AACGCAG59800.026.5716
ACGCAGA71300.021.9957857
CGTGTTA800.00290816321.55244312
CGCAGAG72050.021.243378
GTACATG85450.020.5813871
AGAGTAC71300.020.26473811
GAGTACT31150.019.59438912
TACATGG87550.019.3736652
CGTAACG1258.701055E-519.33292452
CAGAGTA78050.018.82145510
ACATGGG88900.018.6545183
GCAGAGT81600.018.129419
GTGGTAT10750.017.963711
Produced by FastQC (version 0.11.5)