FastQCFastQC Report
Mon 6 Feb 2017
SRR2078259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295976
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.047.8192141
GTATCAA61400.039.2499961
TATCAAC84750.028.428512
ATCAACG85800.028.0750773
TCAACGC87350.027.5768934
CAACGCA88700.027.0399515
AACGCAG92650.025.8123256
ACGCAGA106750.022.1093257
TAGCGTA800.002909382321.55088851
CGCAGAG108700.021.518528
GTACATG82600.020.8221131
AGAGTAC108800.020.26164611
GAGTACT71700.020.06366312
TACATGG83850.020.0101952
CAGAGTA115550.019.31688710
GCAGAGT116750.019.2939559
ACATGGG85150.018.9681363
AGTACTT76850.018.71912213
GTACTTT77500.018.51760914
TACCGTA2255.4315024E-918.4298327
Produced by FastQC (version 0.11.5)