FastQCFastQC Report
Mon 6 Feb 2017
SRR2078258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11450.037.346441
GGTATCA6000.028.7376841
ATCAACG15600.027.409843
TCAACGC15750.027.3677374
TATCAAC16700.026.015962
CAACGCA16450.025.9921155
AACGCAG17000.025.9498336
CAATCGC1001.5526466E-524.17117546
CGTGCAA953.0716116E-421.78250510
ACGCAGA19950.021.767137
GTACATG24150.020.848211
CGCAGAG19900.020.6162178
AGAGTAC18950.020.38398211
GTGCGAA850.00412720220.28762811
TACATGG25250.019.9378012
GTATTAG1651.8350547E-618.8101221
ACTCGAC1301.17279254E-418.5932146
GAGTACT8850.018.31617712
ACATGGG25700.018.2479973
TCGTGCG950.007847751518.15208812
Produced by FastQC (version 0.11.5)