FastQCFastQC Report
Mon 6 Feb 2017
SRR2078258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.13388986961470314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.045.093391
GTATCAA17650.040.8558851
TATCAAC26100.028.0179162
ATCAACG26200.027.9094563
ACCGTTA500.007755506327.58439635
TCAACGC26500.027.331954
CAACGCA27150.026.8052395
AACGCAG28200.025.8071696
GTGCCGA1651.1459633E-1025.09169811
CGTGCCG1704.434696E-922.32423210
ACGCAGA34200.021.1103537
GCGATAC1004.3329844E-420.70065114
GAGTACT23050.020.5060712
CGCAGAG34850.020.4871928
CGCACAA1206.4024585E-520.11798925
AGAGTAC33950.019.8165311
CGATACT1056.024871E-419.71276715
AGTACTT24350.019.69466613
CTTACAC3250.019.193733
GTACATG23450.018.775731
Produced by FastQC (version 0.11.5)