FastQCFastQC Report
Mon 6 Feb 2017
SRR2078257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137927
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.035.5445671
GGTATCA17200.033.679381
ATCAACG44950.026.8454533
TCAACGC44900.026.7223994
TATCAAC46200.026.0408272
CAACGCA45850.025.9413035
AACGCAG47900.024.7527286
GTACATG59300.021.165571
ACGCAGA55300.020.8181367
TACATGG59950.020.2406652
CGCAGAG57300.019.9133668
AGAGTAC53650.019.79725511
GAGTACT28050.019.54736312
ACATGGG61900.019.2159043
CAGAGTA59350.018.24453710
GCAGAGT61950.017.9241499
AGTACTT32650.016.8989913
GTACTTT31350.016.71975914
CATGGGG46400.016.3473454
GTATAGG5550.015.5321031
Produced by FastQC (version 0.11.5)