FastQCFastQC Report
Mon 6 Feb 2017
SRR2078257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137927
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.10402600275874714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.040.8863451
GTATCAA48050.040.286841
TATCAAC65850.029.2811472
TCAACGC68200.027.9618344
ATCAACG69300.027.6180613
CAACGCA69700.027.3103285
AACGCAG72900.026.2542086
ACGCAGA86100.021.9822527
CGCAGAG87300.021.6521848
GAGTACT59850.021.44198812
AGAGTAC85550.021.00893211
TACCGTA1356.76347E-620.4668487
CGCTCGA1356.8323225E-620.44289810
AGTACTT63800.020.2766813
CAGAGTA91300.019.91251410
GTACATG58350.019.6792931
GTACTTT66200.019.22890914
GCAGAGT95750.019.164089
TACATGG59200.018.9212512
GCACCGT3550.018.5572056
Produced by FastQC (version 0.11.5)