FastQCFastQC Report
Mon 6 Feb 2017
SRR2078256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361907
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.036.346461
GTATCAA33850.032.806741
TCAACGC41200.025.8642314
ATCAACG42050.025.5032713
CAACGCA42350.025.1602955
TATCAAC43650.025.1192992
CGATTCG700.001330744524.65402429
AACGCAG44600.023.7288196
ACGCAGA51400.020.3221917
GTACATG67750.019.3946341
CGCAGAG54650.018.9894928
TACATGG67600.018.9740872
AGAGTAC55000.017.74405311
ACATGGG67200.017.703183
GTATAGA7150.016.8821561
GAGTACT25450.016.80243312
GCAGAGT59950.016.6816069
CAGAGTA60300.016.29883810
GTGTATA14350.016.1023331
CGCGCTT1300.002523707515.92218762
Produced by FastQC (version 0.11.5)