FastQCFastQC Report
Mon 6 Feb 2017
SRR2078256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361907
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.041.500621
GTATCAA54900.038.698531
TATCAAC72400.029.1431982
ATCAACG73850.028.4272253
TCAACGC74000.028.2291564
CAACGCA75500.027.5765445
AACGCAG80000.026.0686666
ACGCAGA91800.022.5712687
CGCAGAG94150.022.0559928
GAGTACT62700.021.67691812
AGAGTAC94200.021.12971911
CAGAGTA99750.020.16157710
AGTACTT66600.020.04497713
GCAGAGT102850.019.7199179
GTACTTT68400.019.5679114
GTACATG66450.019.1415631
CGCACGA1651.8383671E-618.80923328
TATACCG1501.6359309E-518.4761315
TACATGG67800.018.3452322
TACTTTT75350.017.76191115
Produced by FastQC (version 0.11.5)