FastQCFastQC Report
Mon 6 Feb 2017
SRR2078255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662286
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37000.035.1337241
GGTATCA17750.033.801461
TATCAAC46900.027.418712
CAACGCA47700.026.4534265
TCAACGC48100.026.3787774
ATCAACG48050.026.1899723
AACGCAG50000.025.2266036
ACGCAGA57900.021.4291157
CGCAGAG60200.020.3264358
GTACGCT1751.4747638E-719.7140360
GTACATG69900.019.4851821
AGAGTAC58100.019.46844711
GAGTACT28050.018.68720412
TACATGG70850.018.68542
CAGAGTA65050.017.81252910
ACATGGG73050.017.6045193
GCAGAGT68100.017.3185929
AGTACTT31000.017.1313913
ATCCCGG5650.017.0971260
CGTGCCG3650.017.00643210
Produced by FastQC (version 0.11.5)