FastQCFastQC Report
Mon 6 Feb 2017
SRR2078255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662286
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.043.526061
GTATCAA49050.039.879131
TATCAAC66850.029.0459462
ATCAACG68950.028.155983
CAACGCA69600.027.6937945
TCAACGC70250.027.6349454
AACGCAG72150.026.7150126
ACGCAGA86000.022.3341947
CGCAGAG88850.021.663758
CGTGCCG4800.021.56184210
GAGTACT58350.021.16605212
GTACATG63650.021.1416511
TACATGG64300.020.8149432
CGCCCAA4150.020.76805750
AGAGTAC87300.020.58871811
CGCGAAC850.004122761520.29311612
AGTACTT61500.020.02584313
CAGAGTA93850.019.62966210
GCAGAGT96300.019.4859379
GTACTTT63250.019.30813814
Produced by FastQC (version 0.11.5)