FastQCFastQC Report
Mon 6 Feb 2017
SRR2078254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198266
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.034.7499851
GTATCAA26450.033.8999981
TCAACGC31950.026.9843064
CAACGCA32300.026.7956225
ATCAACG33500.025.8363653
TATCAAC34600.025.312812
AACGCAG34900.024.6898986
ACGCAGA40050.020.9996387
GTACATG57400.020.7881661
TACATGG58200.020.0251832
CGCAGAG42900.019.5264328
AGAGTAC42000.018.88515911
ACATGGG61200.018.4809783
GCACCGT6550.018.4175326
TATATCG1150.001111660517.99758566
GTGTAGC9000.017.626551
CATGGGG46800.017.0955944
CAGAGTA47400.016.87919610
AAAGCGT5550.016.78191624
CGCAATA1250.001927473916.57780336
Produced by FastQC (version 0.11.5)