FastQCFastQC Report
Mon 6 Feb 2017
SRR2078254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198266
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.041.60051
GTATCAA44000.039.236831
TATCAAC61300.028.0998462
CAACGCA61650.027.9914175
TCAACGC62000.027.83344
ATCAACG62900.027.4902383
AACGCAG65850.026.3639556
ACGCAGA74800.022.996877
CGTGCCG3250.022.29066810
CGCAGAG78200.021.923368
AGAGTAC76450.021.02788411
GAGTACT48950.020.85962112
ACCGTAT2051.7462298E-920.1922578
GTACATG58350.020.0813621
CAGAGTA81800.019.98997110
TATATCG1056.046876E-419.70386766
AGTACTT51900.019.60749213
TACATGG60750.019.2831732
GCAGAGT86900.019.1718989
GTACTTT52550.019.10238814
Produced by FastQC (version 0.11.5)