FastQCFastQC Report
Mon 6 Feb 2017
SRR2078253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731512
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.033.775651
GGTATCA11050.032.4541931
TATACCG1052.3479515E-829.551375
TCAACGC25900.026.8898624
CAACGCA25950.026.8372765
ATCAACG26650.026.2624843
TATCAAC28300.025.3396912
AACGCAG27500.025.1905216
TAGCGCG800.002894405721.56969829
CCGTGAT3200.021.56158655
CGTGATC3050.021.49089256
ACGCAGA33300.020.389587
GTACATG42150.020.3704931
CGCAGAG34050.019.6396488
ATCCCGG3750.019.31583260
TATCCGG900.005754246419.1558810
TACATGG43800.019.0487142
ATACCGT1451.2766446E-519.0149776
ACCGTGC5400.018.5128
GATCCCG4000.018.10911459
Produced by FastQC (version 0.11.5)