FastQCFastQC Report
Mon 6 Feb 2017
SRR2078253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731512
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.039.1154141
GTATCAA31300.038.547371
ATCAACG43000.028.045873
TATCAAC43550.027.8548622
TCAACGC43200.027.6753714
CGTTCGG500.007758725427.583436
CAACGCA43700.027.2794195
AACGCAG46100.025.9344066
CGCACGA2150.025.65897835
ACGCAGA54100.021.955667
CGCAGAG55800.021.3870188
GATCGTT3000.020.69352721
AGAGTAC54100.020.59985511
GCGCACG2950.019.869434
TACATGG42400.019.8637662
GTACATG42400.019.6248021
GAGTACT34950.019.54685812
CTTAGAC4100.019.4402643
CAGAGTA59050.019.16517810
GTACCGA2901.8189894E-1219.119666
Produced by FastQC (version 0.11.5)