FastQCFastQC Report
Mon 6 Feb 2017
SRR2078252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086111
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.034.255241
GTATCAA27100.033.594381
TCAACGC33800.026.5257594
ATCAACG34550.025.9499453
CAACGCA35100.025.5420955
AACGCAG35950.025.4031286
TATCAAC36600.024.587732
ACGCAGA43300.020.456797
CGCAGAG45450.019.2648268
ACGGCGT1108.285049E-418.8028558
GTACATG53750.018.7342531
CGTGCCG3250.018.03924810
AGAGTAC44650.017.91923111
TACATGG55500.017.7677352
CGGTATG2601.7716957E-917.24960959
GTATAAG5350.016.7591321
ACATGGG58200.016.5899943
GAGTACT19550.016.58188612
CAGAGTA49800.016.55089210
GTATAGG5250.016.4214951
Produced by FastQC (version 0.11.5)