FastQCFastQC Report
Mon 6 Feb 2017
SRR2078252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086111
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.040.2464031
GTATCAA38250.036.0665781
CGTGCCG3600.026.83194210
TATCAAC52950.026.246352
ATCAACG53050.026.193723
TCAACGC53500.026.038174
CAACGCA53950.025.6925185
GTGCCGA3900.025.6525211
GCGATAC3400.025.36633314
AACGCAG56250.024.6419836
TGCGATA3750.022.07885613
GTGCGAT4150.021.61333812
GCACCGT5950.021.5487946
ACGCAGA64950.021.117567
GTGGTAT8850.020.7580171
GGTGCGA4050.020.44338611
CGCAGAG67100.020.3067288
AGCACCG7300.019.4625245
ATAGGCG3200.019.39640656
GTACATG57100.019.3645691
Produced by FastQC (version 0.11.5)