FastQCFastQC Report
Mon 6 Feb 2017
SRR2078251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603815
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.14359511539672593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22310.1391058195614831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.034.5411871
TATACCG703.867632E-529.5538235
GTATCAA49950.028.783761
TTAGTCG1701.70985E-1024.32705739
TCGCCCG1151.6923859E-624.00969322
ATCAACG61800.023.0413823
TCAACGC61900.023.0048774
TCGATTA750.001991579523.0035448
CAACGCA62950.022.6759285
TATCAAC63800.022.3710272
GTATAGG3250.022.2783431
GTACATG80550.022.1723211
AACGCAG65350.021.6787766
TACATGG81650.021.407112
ACATGGG84150.020.4043223
CGCTCGT850.004109767320.30358328
TAGTCGG2051.7516868E-920.1856240
TATATCG1206.399952E-520.12055666
CTGGTCG2750.020.0626569
GAGTACT28300.019.25182212
Produced by FastQC (version 0.11.5)