FastQCFastQC Report
Mon 6 Feb 2017
SRR2078251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603815
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.26156383373394065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.15781121887499494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.14920673519077948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31450.038.6588481
GTATCAA72500.037.8876461
ATCAACG98400.027.9390133
TATCAAC98750.027.633072
TCAACGC99100.027.6019084
CAACGCA100150.027.2433785
AACGCAG103850.026.4394456
AACCGTT1851.8189894E-1124.23072234
GAGTACT75200.023.53996512
AGTACTT78950.022.90263413
ACGCAGA120600.022.6460747
AGAGTAC115450.022.47657611
CGCAGAG120900.022.4560188
GTACTTT80000.021.99816514
GTACATG81850.021.8370231
CAGAGTA122850.021.37547310
TACATGG83600.021.2081952
GCAGAGT125700.020.9686059
ACATGGG85350.020.486773
ACTTTTT90550.020.27163716
Produced by FastQC (version 0.11.5)