FastQCFastQC Report
Mon 6 Feb 2017
SRR2078250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066003
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14800.035.418831
GGTATCA7500.035.4064031
TAGGTCG553.2714062E-431.3398255
TCAACGC18150.027.9222454
ATCAACG18550.027.5059993
CAACGCA18750.027.0274645
TATCAAC19150.026.8229622
AACGCAG19550.026.0941436
GTATAGG1951.8189894E-1224.7597221
GTATTCG700.001336769324.6333981
CGTGCCG902.1331037E-422.98901210
GGACGAA750.002003933822.9782247
ACGCAGA22500.022.2122827
CGCAGAG23450.021.3144258
GTACATG28250.020.7530921
GCTCGAC850.004113460420.29967319
TATACAG5500.020.0574875
TACATGG28800.019.870322
AGAGTAC23100.019.70486611
ACATGGG29450.019.4326673
Produced by FastQC (version 0.11.5)