FastQCFastQC Report
Mon 6 Feb 2017
SRR2078250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066003
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.040.565041
GTATCAA20300.037.0476841
CGTATTA450.00463439730.65061228
GCGATAC802.7625701E-630.18585614
TGCGATA906.908418E-626.83187113
TATGCCG1457.075869E-1026.15871443
TATCAAC30050.024.7895452
CGTGCCG700.001333840924.64267310
ATCAACG30400.024.1622223
TCAACGC30650.023.7390524
CAACGCA30800.023.7359335
AACGCAG32700.022.356786
CTCGTAT1554.1085514E-822.24641439
TCGTATG1554.1085514E-822.24641440
ACTGCGT1103.2715478E-521.950268
GTACATG27950.021.5756971
TAGGCCG800.002904161621.55626923
TGCGGCG1304.943633E-621.21965829
TACATGG27850.021.0250032
ACATGGG28700.020.0401383
Produced by FastQC (version 0.11.5)