FastQCFastQC Report
Mon 6 Feb 2017
SRR2078249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428998
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.036.766361
GTATCAA37150.030.8134061
TCAACGC45100.024.5377774
CAACGCA45400.024.3741235
ATCAACG46050.024.2556613
TATCAAC46350.024.1705572
AACGCAG47400.023.4848426
ACGCAGA55800.019.7646087
GTACATG65900.019.6203291
CGCAGAG59200.018.6892388
AGAGTAC55400.018.6723411
TACATGG67100.018.5968362
GAGTACT26350.018.45124812
CGCTACG950.00773850318.19772731
GTGGTAT10200.017.2397291
CAGAGTA61050.017.22667310
ACATGGG71250.017.1283743
AGTACTT29900.016.7218413
GTATAGG5800.016.6452561
GTATAGC6050.016.5273441
Produced by FastQC (version 0.11.5)