FastQCFastQC Report
Mon 6 Feb 2017
SRR2078249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428998
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.044.145361
GTATCAA56750.038.4216081
ATCAACG77050.028.14643
TATCAAC77100.028.0446622
TCAACGC77750.027.8929924
CAACGCA78650.027.573815
AACGCAG82850.026.3014266
ACGCAGA96250.022.4988657
CGCAGAG99350.021.7044878
GAGTACT65900.021.56817212
AGAGTAC96900.021.00534611
AGTACTT68750.020.62389613
GTACTTT69450.020.41602314
CAGAGTA103050.020.12000510
GTACATG67350.019.6100231
CGGCACG3350.019.5615422
GCAGAGT109050.019.4538829
TAACACG1451.2212524E-519.1136284
TACATGG67650.018.7470652
TGCGCGA1301.1863786E-418.56737952
Produced by FastQC (version 0.11.5)