FastQCFastQC Report
Mon 6 Feb 2017
SRR2078248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608802
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.033.1296121
GTATCAA38700.032.9671331
GCGATAC3350.026.76613214
TCAACGC48100.025.9470084
CAACGCA48850.025.5481475
ATCAACG49600.025.2998813
TATCAAC50900.024.7882212
AACGCAG51900.024.1740556
TGCGATA3850.023.29001213
GTGCGAT4550.021.2228512
GTACATG77650.020.471511
ACGCAGA60500.020.4544537
GTGGTAT10900.020.2462161
TACATGG77350.020.0109712
TCGCAAT3500.019.7171549
ACATGGG79450.019.2224433
CGCAGAG64000.019.176118
AGAGTAC60400.018.9561411
CGAATCG2801.2732926E-1118.50899337
CGTGCCG3400.018.25754410
Produced by FastQC (version 0.11.5)