FastQCFastQC Report
Mon 6 Feb 2017
SRR2078248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608802
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.041.2339551
GTATCAA54100.040.485121
TATCAAC72600.029.869022
ATCAACG76000.028.4822583
CAACGCA76650.028.3310955
TCAACGC76200.028.3165994
AACGCAG79900.027.2653986
GCGATAC3350.024.7166114
TGCGATA3600.023.0001813
ACGCAGA94800.022.6544977
TAGGCGC2900.022.59192757
CGTACAC3150.021.9901123
GTACATG71050.021.510481
CGCAGAG99850.021.3515578
TACATGG73300.020.746452
GTGCGAT4000.020.70016112
AGAGTAC97300.020.63633711
GAGTACT63850.020.58669312
ATAGGCG3250.020.1589556
CGATACT4150.019.95119715
Produced by FastQC (version 0.11.5)