FastQCFastQC Report
Mon 6 Feb 2017
SRR2078247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451699
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17800.036.0308571
GTATCAA36750.035.0910151
TATCAAC45600.027.8996542
TCAACGC46250.026.913934
ATCAACG47700.026.456693
CAACGCA46950.026.3630835
AACGCAG49100.025.1323156
ACGCAGA55550.021.6561457
GTACATG65100.020.8158051
CGCAGAG58000.020.3885238
TACATGG67350.019.2992782
AGAGTAC56850.018.56394211
ACATGGG67700.018.3861713
CAGAGTA61400.017.58146910
GAGTACT25900.017.31137812
GCAGAGT64950.016.8860159
GTATATA8300.016.617421
GTGGTAT9600.016.5222151
GTATAGG6000.016.0912021
GCGCGTT2402.2817403E-715.83068736
Produced by FastQC (version 0.11.5)