FastQCFastQC Report
Mon 6 Feb 2017
SRR2078247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451699
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44250.040.3421671
GGTATCA18300.037.882981
TATCAAC60850.029.104112
ATCAACG61850.028.519733
TCAACGC61800.028.2624234
CAACGCA61900.028.2167635
AACGCAG65250.027.0336446
ACGCAGA76750.022.6749697
CGCAGAG79700.021.7268458
CGTACAC2401.8189894E-1221.6594453
CGTGCCG4250.021.1047410
AGAGTAC78050.020.8182211
GTACATG61500.020.7413941
GAGTACT50400.020.46573612
CGGCACG4300.020.05132722
GCACCGT6250.019.9613466
TACATGG62600.019.4877782
CAGAGTA84450.019.48562410
TCGGCAC4650.019.2837721
AGTACTT53900.019.264813
Produced by FastQC (version 0.11.5)