FastQCFastQC Report
Mon 6 Feb 2017
SRR2078246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641360
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.034.995121
GTATCAA25550.034.2780571
ATCAACG33350.026.0526333
TCAACGC33200.025.9642244
TATCAAC34700.025.4326252
CAACGCA34150.025.0361845
AACGCAG36800.023.5064666
TCGCGTC1253.49133E-622.08975417
TTGCGCG800.002890993421.57400118
GTACATG58350.020.8596611
TACATGG60000.020.0518682
ACATGGG60550.019.7019353
ACGCAGA43050.019.6947237
TCGACAG2505.0931703E-1119.32499548
TAGACGC900.005754214719.1558254
CTACGAA900.005754214719.1558251
CGCAGAG44350.018.8077228
TATAACG950.0078711218.1437512
GCGCACT2701.5279511E-1017.88368442
CATGGGG38700.017.6411784
Produced by FastQC (version 0.11.5)