FastQCFastQC Report
Mon 6 Feb 2017
SRR2078246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641360
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.042.9453851
GTATCAA36300.038.89111
TATCACG500.007760596527.58195331
TATCAAC51650.027.456332
ATCAACG51900.027.1203293
TCAACGC53100.026.5074394
CAACGCA54400.025.873995
AACGCAG55800.025.286956
GTGCGAT1151.6985941E-623.9996612
TGCGATA1202.4592064E-622.99967413
CGGCACG3000.021.84303322
TCGGCAC3200.021.55562421
ACTCGAC2251.0913936E-1121.45263146
ACGCAGA65600.021.2719387
GAGTACT39100.021.08793612
GCGATAC1154.604674E-520.99970214
CGCAGAG67350.020.6408428
ACGCCCA2900.020.21091549
GTACATG55600.019.9635451
TACATGG54900.019.9574132