FastQCFastQC Report
Mon 6 Feb 2017
SRR2078245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019025
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.033.2006231
GTATCAA27350.032.397571
TCAACGC32400.026.2818744
CAACGCA32600.025.908495
ATCAACG33250.025.7130533
AACGCAG33750.024.9155066
GCGATAC2100.024.63233414
TATCAAC35150.024.5180952
TGCGATA2251.0913936E-1121.457513
CGTGCCG2750.021.31816710
ACGCAGA40050.020.9956917
CGCAGAG41200.020.0785458
GTACATG46250.019.5310781
TACATGG46350.018.8910472
ACGGCGT1108.2138815E-418.82695819
GGTGCGA3200.018.320311
AGAGTAC40250.018.1636711
GAGTACT18750.018.024312
TCAATCG2501.0568328E-917.93278345
ACTCGAC1753.1952204E-617.7515746
Produced by FastQC (version 0.11.5)