FastQCFastQC Report
Mon 6 Feb 2017
SRR2078245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019025
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34250.039.1671871
GGTATCA14500.036.814991
TATCAAC46200.028.9533182
TCAACGC46400.028.8988934
CAACGCA46250.028.7678685
ATCAACG46200.028.7240053
AACGCAG48300.027.3316636
ACGCAGA56400.023.2696557
CGATCGT1103.2808122E-521.94384442
CGCAGAG60250.021.638558
GTACATG48600.021.3258761
AGAGTAC58250.020.7287511
TCGATCG1206.414151E-520.1151941
TACATGG49900.020.0701372
GAGTACT36300.019.67273312
CAGAGTA64250.019.22254610
ACATGGG50450.019.1615773
CGTGCCG3250.019.10689710
GCAGAGT67050.018.67439
CGAATCG3200.018.31828337
Produced by FastQC (version 0.11.5)