FastQCFastQC Report
Mon 6 Feb 2017
SRR2078244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502769
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.036.62191
GTATCAA38100.032.40511
TCAACGC48450.024.6937314
ATCAACG48950.024.651823
TATCAAC49700.024.6251872
CAACGCA49400.024.148095
AACGCAG52300.022.8663256
GTATACG1103.2784184E-521.9462681
TGCGATA2001.2914825E-920.68846913
GTACATG76350.020.1456431
TACATGG76500.019.5134492
ACGCAGA62400.019.111127
ACATGGG80200.018.3133953
GTATTAC5150.018.0805591
CGCAGAG65650.018.0621938
GCGATAC2307.3268893E-917.98997314
GTGTAGC11000.017.5570131
AGAGTAC62800.016.91095511
ACCGTGC6550.016.314258
CGTGCCG3401.4551915E-1116.2262510
Produced by FastQC (version 0.11.5)