FastQCFastQC Report
Mon 6 Feb 2017
SRR2078244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502769
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.043.723671
GTATCAA51750.038.9963951
TATCAAC70550.028.397292
ATCAACG72450.027.5541213
TCAACGC73000.027.4889534
CAACGCA73650.027.2463475
AACGCAG77200.026.0832256
ACGCAGA92550.021.5728977
GTACATG73550.020.6020581
CGCAGAG96750.020.573938
AGAGTAC93500.020.25475911
TACATGG75650.019.8850572
CAGAGTA99550.019.33567410
ATGCGCG900.00575555119.15553551
GAGTACT61750.018.82567812
ACATGGG78350.018.7112943
GCAGAGT104150.018.5821519
TAGACCG2104.7870344E-818.1541465
AGTACTT65200.017.82953513
GTACTTT66000.017.45662314
Produced by FastQC (version 0.11.5)