FastQCFastQC Report
Mon 6 Feb 2017
SRR2078243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348866
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31500.031.9682061
GGTATCA17650.030.0899851
TCAACGC38000.025.315314
CAACGCA38800.024.9694795
ATCAACG39450.024.3848363
TATCAAC41250.024.0704862
AACGCAG42200.022.9494046
GTACATG59650.019.888131
ACGCAGA48650.019.7655147
CGTATAC1056.049565E-419.7026463
CGCAGAG51450.018.625638
TACATGG62300.018.0957282
GCACCGT5150.018.0689966
ACATGGG61150.017.3102993
GTGCCGA3205.456968E-1217.24128711
AGAGTAC49500.017.20645711
CGTGCCG2904.2382453E-1016.6467610
GGACCGT2502.064371E-816.543176
CAGAGTA54450.016.46550810
GTGGTAT10450.016.1705841
Produced by FastQC (version 0.11.5)