FastQCFastQC Report
Mon 6 Feb 2017
SRR2078243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348866
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.041.3208161
GTATCAA46000.039.7102281
TATCAAC64300.028.023422
ATCAACG65450.027.6345543
TCAACGC65350.027.5707994
CAACGCA66550.026.9695265
AACGCAG69350.025.7807066
ACGCAGA80200.021.8808217
GTACCGT1755.9080776E-921.7794156
GCGATAC2101.0186341E-1021.35849614
CGCAGAG82650.021.0354128
GAGTACT58100.020.54647412
TGCGATA2201.9099389E-1020.38765513
TGCGTCG850.0041204420.2949710
AGAGTAC82200.019.85302411
GTACATG57350.019.7635021
CAGAGTA87950.018.86889810
AGTACTT63600.018.66155813
GTACTTT64150.018.60912714
GCAGAGT91200.018.5338029
Produced by FastQC (version 0.11.5)