FastQCFastQC Report
Mon 6 Feb 2017
SRR2078242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285598
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17350.038.5549431
GGTATCA6600.032.391091
TCAACGC21150.030.6496354
CAACGCA21650.029.7825285
TATCAAC22500.029.7255152
ATCAACG22200.029.198853
AACGCAG23400.027.6982366
CGACCGC700.001335525724.63780610
ACGCAGA27600.022.9836467
CGCAGAG28950.021.794488
AGAGTAC27600.021.1206711
GTACATG33400.020.7504461
GAGTACT13300.020.74762712
GTACCGA1851.1863449E-820.4989286
CGCCCAA1356.7859455E-620.45778850
TACATGG34000.019.8740922
CGTGCCG2102.3555913E-919.71024510
CAGAGTA31250.019.20566410
ACATGGG34150.018.9813923
GCAGAGT32550.018.8624929
Produced by FastQC (version 0.11.5)