FastQCFastQC Report
Mon 6 Feb 2017
SRR2078242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285598
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10150.038.938691
GTATCAA24500.038.772791
ATCAACG33650.027.8046533
TATCAAC33900.027.6052
TCAACGC34100.027.4377294
CAACGCA34550.027.2809585
AACGCAG35900.026.2550746
CGTGCCG1803.43789E-1022.99850810
CGCGTTG750.001999321422.98777637
GCACCGT2950.022.3187756
ACGCAGA42400.022.1565067
TATACCG952.975619E-421.8860025
GCGCGTT800.002908576721.55103736
TATCGGT800.002909233321.550250
CGCAGAG44050.021.4524698
AGAGTAC42800.020.87598211
AGCGCGT850.004133147720.2833335
GTACATG31550.020.2190631
GAGTACT29750.020.17684212
ACCGTGC4150.019.945088
Produced by FastQC (version 0.11.5)