FastQCFastQC Report
Mon 6 Feb 2017
SRR2078241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501324
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.038.146211
GTATCAA42150.034.76461
ATCAACG52400.027.4356923
TCAACGC52400.027.3041064
CAACGCA54400.026.4259665
TATCAAC56550.025.6630612
AACGCAG57150.025.2081436
ACGCAGA66300.021.31467
CGCAGAG68850.020.326968
GTACATG71800.020.1683671
TACATGG74150.019.339322
AGAGTAC67450.019.07192411
CAGAGTA73200.018.1862810
GTGGTAT9950.018.0188121
ACATGGG79650.017.659793
GAGTACT31150.017.6038112
GCAGAGT77400.017.5558919
GTACTAG3800.017.2391531
GTATAAG7750.016.460611
CGGTTAC2109.943305E-716.43534728
Produced by FastQC (version 0.11.5)