FastQCFastQC Report
Mon 6 Feb 2017
SRR2078241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501324
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.10386499309965441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.043.40251
GTATCAA60200.040.366461
GTTCGCG400.00259821934.4964716
ATCAACG83300.028.9145723
TATCAAC83900.028.7531432
TCAACGC84750.028.2971944
CAACGCA87650.027.5191045
AACGCAG91150.026.3103356
ACGCAGA105600.022.6591557
CGCAGAG108900.021.7609318
TCGATCG1154.6239817E-520.98903741
GAGTACT71450.020.76189212
AGAGTAC108100.020.58423411
TTCGCGC850.0041330420.28433217
GTACATG75750.020.1358151
AGTACTT74600.019.97770713
CAGAGTA115800.019.54321710
GTACTTT76400.019.41671814
CGTGCCG3200.019.4054310
GCAGAGT119350.019.2490439
Produced by FastQC (version 0.11.5)